Fig. 4.
GSNO treatment suppresses histone H4K12 acetylation elevated by TNBS inflammation. Histone H4 acetylation at lysine residues 8 (A), 12 (B), and 16 (C) increased significantly in colonic muscularis externae of TNBS rats. N = 4. *Significantly different vs. Ctr (P < 0.05). D: inflammation increased histone H4K12 acetylation at the NF-κB binding region of rat Icam-1 promoter and GSNO significantly suppressed it. We quantitated Icam-1 promoter precipitation by anti-acetylated H4K12 using real-time PCR and normalized to input. Rabbit IgG served as negative control. Values are means ± SE; n = 3. *Significantly different vs. Ctr. (P < 0.05). #Significantly different vs. TNBS (P < 0.05).