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. 2012 Apr 19;303(1):G103–G110. doi: 10.1152/ajpgi.00381.2011

Fig. 7.

Fig. 7.

Trichostatin A (TSA) dramatically elevates ICAM-1 protein expression and histone H4 acetylation. Colonic muscularis externae from naive adult rats were incubated with 1 μM TSA for 24 h and snap-frozen in liquid nitrogen. Whole cell lysates were prepared for immunoblotting. ChIP-qPCR assays were performed with indicated antibodies, SYBR Green Master Mix (Applied Biosystems), and primers specific to the NF-κB binding area (−290/−127) of the rat Icam-1 promoter. Rabbit IgG was used for mock precipitation. A: TSA markedly increased ICAM-1 protein expression and global H4K8Ac, H4K12Ac, and H4K16Ac in rat colonic muscularis externae. B: TSA enhanced p65 binding to the Icam-1 promoter. It also increased histone H4K8Ac, H4K12Ac, and H4K16Ac associated with the Icam-1 promoter. In addition, TSA significantly attenuated HDAC3 association with the Icam-1 promoter. N = 3. *Significantly different vs. Ctr (P < 0.05).