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Removal of Cl− ion from bath solutions eliminates both basal and cAMP-stimulated Isc as well as their responses to TTX and R-568. Shown are representatives of 2–6 recordings of Isc responses of distal colon to bilateral Cl− ion substitution and subsequent additions of serosal bumetanide (A), serosal TTX (B), bilateral forskolin (D), mucosal amiloride (A–D), and serosal R-568 with (D) or without (C) prior forskolin stimulation. Similar responses were noted in the proximal colon as well.
