Table 5.
Hepatic gene expression levels of high and low responders on the HCHF diet for 24 wk
| Group and Gene Symbol | High Responders | Low Responders | P Value |
|---|---|---|---|
| Cholesterol metabolism | |||
| HMGCR | 101 ± 20 | 41 ± 5 | 0.02* |
| LDLR | 43 ± 7 | 102 ± 24 | 0.04* |
| SRBI | 77 ± 4 | 88 ± 14 | 0.43 |
| CYP7A1 | 33 ± 6 | 78 ± 22 | 0.09 |
| CYP27A1 | 31 ± 2 | 90 ± 8 | <0.001* |
| Bile secretion | |||
| ABCG5 | 78 ± 5 | 85 ± 11 | 0.54 |
| ABCG8 | 37 ± 4 | 103 ± 9 | <0.001* |
| NPC1L1 | 34 ± 4 | 85 ± 11 | 0.002* |
| ABCB11 | 85 ± 8 | 123 ± 31 | 0.27 |
| ABCB4 | 64 ± 3 | 105 ± 14 | 0.02* |
| Transcription factors | |||
| SREBP2 | 43 ± 2 | 121 ± 14 | <0.001* |
| FXR | 66 ± 6 | 70 ± 9 | 0.76 |
| LXRA | 69 ± 7 | 56 ± 3 | 0.11 |
| Fatty acid metabolism | |||
| FASN | 65 ± 4 | 86 ± 41 | 0.63 |
| CPT1 | 20 ± 2 | 25 ± 6 | 0.40 |
| PPARA | 22 ± 3 | 32 ± 6 | 0.19 |
| CD36 | 58 ± 7 | 85 ± 10 | 0.06 |
| Inflammation | |||
| TNF | 69 ± 7 | 10 ± 3 | <0.001* |
| NFKB1 | 18 ± 1 | 6 ± 1 | <0.001* |
| COX2 | 67 ± 13 | 21 ± 11 | 0.026* |
| Oxidative stress | |||
| CYBA | 21 ± 3 | 2.5 ± 0.4 | <0.001* |
| NCF1 | 63 ± 5 | 13 ± 4 | <0.001* |
| Collagen synthesis | |||
| TGFB1 | 61 ± 7 | 13 ± 2 | <0.001* |
| PDGFA | 64 ± 9 | 22 ± 11 | 0.02* |
| PDGFB | 61 ± 7 | 7 ± 1 | <0.001* |
| Col1A1 | 18 ± 1 | 0.15 ± 0.07 | <0.001* |
| Col3A1 | 18 ± 3 | 0.65 ± 0.16 | <0.001* |
| Col4A1 | 19 ± 2 | 1.8 ± 0.3 | <0.001* |
Values are means ± SE, N = 5 opossums in each group. Gene expression was measured by real-time PCR.
*
Significant difference between high and low responders.