Fig. 1.

Secondary structure of calcineurin homologous proteins CHP1–CHP3 and their relationship with other EF-hand Ca²⁺-binding proteins. A: alignment of amino acid sequences of vertebrate CHP1–3 demonstrating conserved domains across vertebrates. Conservative secondary structural elements are emphasized, myristoylation site (black); EF1 and EF2 hands, which do not bind Ca²⁺ (yellow); EF3 and EF4 that bind Ca²⁺ (blue); nuclear export signal (NES; green); flexible linker (violet). The N- and C-lobes are also shown. B: phylogenetic tree of EF-hands Ca²⁺-binding protein family based on amino acid alignment. Phylogenetic trees were obtained using the Bayesian approach, which is implemented in MrBayes (125). Numbers in the nodes represent the posterior probability. Hs = Homo sapiens; Pt = Pan troglodytes; Mm = Mus musculus; Rn = Rattus norvegicus; Mmu = Macaca mulatta; Dr = Danio rerio; Bt = Bos taurus; Xl = Xenopus laevis; Gg = Gallus gallus; Md = Monodelphis domestica, Oc = Oryctolagus cuniculus; Xt = Xenopus tropicalis.