Fig. 7.

αC664A mutation does not alter the removal of the αT663 variant from the oocyte plasma membrane. Oocytes were injected with either wild-type channels (αT663/C664) or the αC664A mutant (αT663/C664A). After 24–36 h, amiloride-sensitive currents at −60-mV holding potential were determined by TEV, and the oocytes were subsequently incubated in a NaCl bath solution without or with 5 μM BFA as indicated. Amiloride-sensitive currents were again determined by TEV after 4 h of incubation without or with BFA. Amiloride-sensitive currents were expressed relative to the amiloride-sensitive current before incubation with BFA (means ± SE). Data were derived from 2 different batches of oocytes, (n = 9–10 oocytes no addition, 18 oocytes with BFA). After 4-h incubation with BFA, there was no significant difference in the reduction of current between wild-type and mutant channels.