Fig. 8.

Trafficking of αT663 variant to the plasma membrane is not altered by the αC664M mutation. Oocytes expressing either wild-type channels (αT663 variant, ●) or the αC664M mutant (○) with a γG536C mutation were treated with MTSET to covalently label and inhibit surface channels. The recovery of benzamil-sensitive currents at −60 mV was plotted as a function of time over 10 min. Benzamil (100 μM) was added at the end of the experiment. Currents were normalized to the benzamil-sensitive current before MTSET treatment. There was no significant difference in the rates of current recovery after MTSET treatment between wild-type and αC664M channels (n = 14–15 oocytes/group from 2 different batches of oocytes).