Fig. 3.

Effects of NSC95397 [an MAPK phosphatase (MKP-1/3) inhibitor] on hypotonic-stress-induced ERK dephosphorylation. A: after pretreatment with NSC95397 of several concentrations for 1 h, A6 monolayers were exposed to a hypotonic culture medium containing NSC95397 at the same concentrations for 2 h. Then, phosphorylated ERK and total ERK were detected by immunoblotting with anti-phospho-ERK and anti-ERK antibodies. B: relative amounts of phosphorylated and total ERK proteins are shown in Fig. 2A. Equal loading of cell lysate to each well was confirmed by measuring GAPDH as an internal control. Values are means ± SE; n = 3. *P < 0.05.