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Effects of PD98059 on ENaC mRNA expression induced by hypotonic stress. After pretreatment with 50 μM PD98059 for 1 h in an isotonic culture medium, A6 monolayers were exposed to an isotonic or a hypotonic culture medium with or without 50 μM PD98059 for 20–24 h. The mRNA expression of α-, β-, and γ-ENaC was detected by real-time PCR. Relative amounts of ENaC mRNA expression were normalized to β-actin. Values are means ± SE; n = 4–7. *P < 0.05.
