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. Author manuscript; available in PMC: 2012 Jul 25.
Published in final edited form as: J Immunol. 2011 Apr 11;186(10):5938–5948. doi: 10.4049/jimmunol.1002635

Figure 8. Reconstitution of TLR7−/− mice with TLR7+/+ but not TLR7−/− BM-derived pDC recapitulates host defense and accelerates viral clearance.

Figure 8

Purified Flt3L-expanded pDC from TLR7+/+ and TLR7−/− bone marrow cells were adoptively transferred to TLR7−/−recipients. Mice were inoculated with PVM 2 h later and endpoints measured at 7 dpi (A and B). The left lung lobe was mechanically dispersed and (A) neutrophils (FSclowGr-1highCD11b+ CD11c−) and NK cells (CD3−CD49b+) identified by flow cytometry. (C and D) CCL3 and TNF-α protein expression in right lung lobe homogenates was measured by ELISA. (E) Representative histogram of lung CD3+CD4+Sca-1+ T cells following transfer of TLR7−/−(top panel) and TLR7+/+ (bottom panel) pDC to TLR7−/− mice. Graphs represent the percent CD3+CD4+ and CD3+CD8+ T cells expressing Sca-1 as detected by flow cytometry. (F) Recipient TLR7−/− mice were weighed at 7 days of age immediately prior to inoculation with PVM (day 0). Mice were weighed every 24 hours thereafter until euthanasia, and weight expressed relative to day 0. (G) Virus recovery in the lungs was measured by qRT-PCR.