Table 1.
Quantitative realtime polymerase chain reaction parameters
| Gene | Forward primer (5′ –3′) | Reverse primer (5′ –3′) | Product size (bp) | Annealing temperature (°C) | Accession # |
|---|---|---|---|---|---|
| IGF-I | TCGCATCTCTCTTCTATCTGGCCCTGT | GCAGTACATCTCCAGCCTCCTCAGA | 240 | 62 | M30653 |
| IGF-II | GACCGCGGCTTCTACTTCAG | AAGAACTTGCCCACGGGGTAT | 203 | 62 | X55638 |
| IGFBP-1 | TGATGACCGAGTCCAGTGAG | GTCCAGCGAAGTCTCACA | 248 | 62 | X54979 |
| IGFBP-2 | CAATGGCGAGGAGCACTC | TGGGGATGTGTGTAGGGAAT | 335 | 55 | S44612 |
| IGFBP-3 | GGCATATTTGAGCTCCACA | TCTCAGAGCACAGACACCCA | 337 | 54 | M76478 |
| IGFBP-4 | TGTGCGTGTGTGTTGATG | GAGGGAGCCAAGATGAGT | 229 | 62 | S77394 |
| IGFBP-5 | TCGTGCGGCGTCTACACTGAG | GAGTAGGTCTCCTCCGCCATC | 203 | 61 | EU727460 |
| IGFBP-6 | AGGAGAGTAAGCCCCAAGCAG | GTCACAATTGGGCAGGTAGAG | 221 | 62 | EU862545 |
| IR | CAGCTGAGCTAGAAGCCAAC | CCATTTCAGCAAGATCTTGTC | 416 | 57 | AY157728 |
| IGF-1R | AACTGTCATCTCCAACCTC | CAAGCCTCCCACTATCAAC | 490 | 53 | X54980 |
| IGF-2R | GAAGAGCAACGTGCACGA | TTGTCCAGGGAGATCAGCA | 368 | 54 | AF342811 |
| FGF7 | TCAGGACAGTGGCTGTTGGAA | CAAACATTTCTCCTCCGCTGTG | 195 | 65 | EU626202 |
| FGF10 | CCCTCGATCAGGACATGGTG | AAGGCAAGGAGACGGACGAG | 87 | 65 | AF213396 |
| FGFR2IIIb | CCGAGTGCTTCAGAACCTTGA | AGAGGCGATGTGGAGTTTGTC | 136 | 65 | DQ908953 |
| PDX-1 | TTTCCCGTGGATGAAGTCTAC | CGGTGCGTGTCCGCTTGTTCT | 101 | 60 | JF728303 |
| Glut2 | CTTTGCAGTTGGTGGAATGAT | GCTGATGAAGAGCACCGATAG | 272 | 60 | AJ318925 |
| Insulin | TCAGCAAACAGGTCCTCGCAAG | GGGCCAGGTCTAGTTACAGTAG | 352 | 60 | U00659 |
| β-Actin | GGGACCTGACCGACTACC | GACAGCGAGGCAGGATGG | 502 | 62 | U39357 |
| S15 | ATCATTCTGCCCGAGATGGTG | TGCTTTACGGGCTTGTAGGTG | 134 | 60 | AY949774 |
Glut2, glucose transporter 2; FGF, fibroblast growth factor; FGFR2IIIb, fibroblast growth factor receptor 2IIIB; IGF-I, insulin-like growth factor I; IGF-II, insulin-like growth factor II; IGFBP, insulin-like growth factor binding protein; IGF-1R, insulin-like growth factor type 1 receptor; IGF-2R, insulin-like growth factor type 2 receptor; IR, insulin receptor; PDX-1, pancreatic and duodenal homeobox 1