. Author manuscript; available in PMC: 2013 Jul 4.

Published in final edited form as: J Am Chem Soc. 2012 Jun 25;134(26):11006–11011. doi: 10.1021/ja304169n

Table 2.

Unzippping rate constants (k) at -140mV for the Gh duplexes formed with 17-mer probe, 13-mer 5’-truncated probe or 13-mer 3’-truncated probe^a


Probe	Rate Constants (s^-1)
	3’ entry		5’ entry
	k₁	k₂	k₁	k₂
17-mer Probe	550 ± 30	930 ± 50	790 ± 40	280 ± 30
13-mer 5’-Truncated Probe	1690 ± 130	880 ± 50	790 ± 70	1000 ± 170
13-mer 3’-Truncated Probe	490 ± 30	3270 ± 440	1603 ± 200	190 ± 20

The 8-bp duplex subsections of the same sequence and their corresponding k are highlighted using the same colors (blue or pink). The k values for unzipping of subsections are assumed to be the same regardless if the subsection is in the 65-mer:17-mer duplex or the truncated duplex with the 13-mer.