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(online color at: www.biophotonics-journal.org) Phantom study to evaluate the effect of water and blood on VPA imaging. (A) Schematic of the constructed phantom. PE: polyethylene. (B) VPA spectra of PE at different water layer thickness. (C) PA amplitude ratio between the first and second overtone excitation as a function of water layer thickness. (D) PA amplitude as a function of blood layer thickness.
