Fig. 4.
CFTR is required but is not rate limiting for cAMP-stimulated liquid transport in pig T2AECs. A: basal and cAMP-stimulated (forskolin+IBMX) liquid transport (Jv) was examined in CFTR+/+ (●), CFTR+/− (▲), and CFTR−/− (○) T2AECs cultured at the ALI for 6–7 days. *P < 0.05 compared with cAMP-stimulated CFTR+/+ and CFTR+/−. B: cAMP-stimulated liquid transport was examined in CFTR−/− T2AECs infected with either control adenovirus (Ad-GFP, ●) or adenovirus encoding pig CFTR (Ad-CFTR, ○). *P < 0.05 compared with Ad-GFP. C: basal and cAMP-stimulated liquid transport was assessed in CFTR+/+ T2AECs after infection with either Ad-GFP or Ad-CFTR. Data are shown as means ± SE; n = 4–6.