. 2012 May 23;303(2):R218–R235. doi: 10.1152/ajpregu.00089.2012

Table 1.

Training and testing schedule

Sessions	Phase	Timeout, s	Limited Hold, s^a	Stimuli	Schedule
6	Spout training	None	None	water	Constant
6	Side training	None	180	0.6 M NaCl or water	Constant
6	Alternation^b	10, 20, 30^c	15	0.6 M NaCl or water	Alternated after x correct responses
31	Discrimination training	30	15	0.6 M NaCl or water	Semirandom^d
25	Alternation^e	10, 20, 30	15	0.6 M NaCl or water	Alternated after x correct responses
10	Discrimination training	30	15	0.6 M NaCl or water	Semirandom
28	Detection testing	30	10	NaCl or water	Semirandom
40-42^f	Discrimination training	30	10	1.0 M sucrose or water	Semirandom
23	Detection testing	30	10	sucrose or water	Semirandom
8	Stimulus control	30	10	0.6 M NaCl or water	Semirandom
23	Discrimination training	30	10	32% Polycose or water	Semirandom
25	Detection testing	30	10	Polycose or water	Semirandom
10	Detection testing II^g	30	10	Polycose or water	Semirandom
10	Stimulus control	30	10	0.6 M NaCl or water	Semirandom
34^h	Discrimination training	30	10	1.0 M maltose or water	Semirandom
23	Detection testing	30	10	maltose or water	Semirandom
10	Stimulus control	30	10	0.6 M NaCl or water	Semirandom
10	Discrimination training	30	10	2.0 M glucose or water	Semirandom
20	Detection testing	30	10	glucose or water	Semirandom
9	Stimulus control	30	10	0.6 M NaCl or water	Semirandom
10	Discrimination training	30	10	1.0 M sucrose or water	Semirandom
20	Detection testing	30	10	sucrose or water	Semirandom

^{^a}

Limited hold is defined as the amount of time the animal had to respond on a reinforcement ball after the sample phase.

^{^b}

A stimulus was presented repeatedly until a predetermined number of correct responses was made. It was not necessary that the correct responses be successive.

^{^c}

On the first 2 days of alternation training, the timeout was 10 s, which was increased to 20 s for days 3 and 4 and increased again to 30 s for days 5 and 6.

^{^d}

Three reservoirs of water and three reservoirs of a taste solution were presented in randomized blocks.

^{^e}

Until this phase, the sample solution delivered to the sample ball following the animal's trial initiation was insufficient to coat the ball. Priming volume was increased. Also, from alternation day 7, volume per lick was increased from ∼1 μl to 1.6 μl and then returned to ∼1 μl from discrimination day 3 onward.

^{^f}

Because of apparatus problems, some mice developed side biases, and thus, they were reintroduced to “side-training” for 1 or 2 sessions.

^{^g}

Lick volume was increased from ∼1 to ∼1.6 μl, sample licks from 5 to 7 licks, reinforcement licks from 15 to 20 licks during this phase.

^{^h}

Maltose powder was out of stock, so animals were given ad libitum food and water for 2 wk and not trained between sessions 10 and 11 of maltose discrimination training.