Fig. 4.

mTOR overexpression preserves cardiac function and prevents cardiac injury during ex vivo I/R. A: LV developed pressure (LVDP) profiles during I/R in WT and mTOR-Tg mice. n = 28 WT mice and 19 mTOR-Tg mice. B: maximum LVDP recovery (percentage of baseline) measured at 40 min of reperfusion. n = 28 WT mice and 19 mTOR-Tg mice. C and D: activity of creatine kinase (CK) and lactate dehydrogenase (LDH) in the effluent collected during the reperfusion period. To determine enzyme activities immediately after ex vivo I/R injury, effluents from hearts exposed to either 20 or 40 min of global ischemia were collected at control perfusion (baseline) and after 40-min reperfusion (I/R). n = 7 WT mice and 8 mTOR-Tg mice in hearts subjected to 20-min ischemia, and n = 3 WT mice in hearts subjected to 40-min ischemia.