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. 2012 Apr 11;120(7):1029–1035. doi: 10.1289/ehp.1104689

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BPA, BPAF, and Zea regulate expression of the ER target genes PR, GREB, MCM3, and SPUVE in Ishikawa/ERα cells. Ishikawa/ERα cells were treated with vehicle (control), 10 nM E₂, or 100 nM BPA, BPAF, or Zea for 18 hr and total RNA was extracted; mRNA levels of PR, GREB, MCM3 and SPUVE were quantified by real-time PCR. Results are presented as fold change (mean ± SEM) relative to the control for three independent experiments. *p < 0.05 compared with control.