Chloride contents and analysis of Cl− fluxes at the plasma membrane and tonoplast resolved over the diurnal Reference Cycle as described in Figure 1 (12 h light:12 h dark, indicated by bars above). Shown are total cytosolic and vacuolar [Cl−] (A), the net flux of Cl− across the plasma membrane and tonoplast (B), the flux of Cl− through the Cl−-permeable transporters at the plasma membrane, comprising the SLAC and R- (ALMT-)type anion channels and H+-Cl− symporter (C), and the flux of Cl− through the Cl−-permeable transporters at the tonoplast, comprising the VCL channel and CLC H+-Cl− antiporter (D). Note the difference in scales between C and D. Note that positive flux is defined as movement of the ionic species (not the charge) out of the cytosol, either across the plasma membrane or the tonoplast.