Fig. 4.

Repression of the BT0366-associated PUL depends on fructose and BT1754.

A. Relative expression levels of genes from the BT0366-associated PUL (BT0360, BT0365) in Bt or Bt-ΔBT1754 strains grown in MM-fructose, MM-arabinan or MM-fructose + arabinan, as determined by qRT-PCR. Values displayed are mean fold changes in expression relative to Bt grown in MM-fructose. Error bars are ± SE. *P < 0.05. The repression of the BT0366-associated PUL that depends upon fructose and BT1754 and its loss upon deletion of BT1754 is noted with arrows.

B. Monosaccharide content of media at various time points after inoculation with Bt (left panel) or Bt-ΔBT1754 (right panel) grown in MM-fructose + arabinose, as determined by HPAEC-PAD. Values represent concentration of the indicated sugar remaining in supernatants collected from growths at specified time points in molar (M).