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Segmentation of organelles based on Linear Absorption Coefficient (LAC) values. (A) A representative diploid cell shown in an orthoslice (i.e., a single slice of tomographic data) and individually segmented organelles; scale bar, 1 μm. (B) LAC values for each organelles. (C) Five different vacuolar compositions found in tomographic data (left), schematic views (middle), and LAC values (right).
