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. Author manuscript; available in PMC: 2013 Apr 2.
Published in final edited form as: Mol Pharm. 2012 Mar 16;9(4):862–873. doi: 10.1021/mp200400s

Table 3.

Apparent Kinetic Parameters of Metabolism of 7HF, 3,7DHF, 5,7DHF, 7,4DHF by SULT1A3

Kinetic parameters 7HF 7,4’DHF 3,7DHF 5,7DHF
Km1 (µM) 2.80 3.42 1.85 2.73
Vmax-1
(nmol min−1 mg−1)		 85.11 20.83 79.7 87.77
Vmax-1/ Km1
(mL min−1 mg−1)		 30.40 6.09 43.0 32.15
Km2 (mM) 1E-06
Vmax-2
(biphasic, nmol min−1 mg−1)		 17.38
Vmax-2/ Km2
(mL min−1 mg−1)		 1.74E07
Ksi 23.12 13.81
R2 0.996 0.994 0.994 0.992
AIC 32.50 3.44 43.68 48.76
Model Substrate
inhibition		 Substrate
inhibition		 Sigmoidal
kinetics of Hill
equation		 Biphasic
kinetics

Kinetic parameters were obtained from using substrate inhibition, sigmoidal kinetics of Hill equation, and biphasic enzyme kinetics models as described under “Materials and Methods”.