Table 1.
Electrophysiological characteristics of pyramidal cells from different clusters
| Characteristics | RS_LRi | RS_HRi | IM | LTS | F Ratio | P Value | Fisher's LSD |
|---|---|---|---|---|---|---|---|
| RMP, mV | −73.2 ± 1.1 | −71.3 ± 1.9 | −71.8 ± 1.0 | −71.7 ± 1.1 | 0.5 | 0.70 | (1,2,3,4) |
| Rin, MΩ | 174 ± 16 | 332 ± 28 | 118 ± 7 | 261 ± 42 | 18.1 | <0.001 | (3); (1); (2,4) |
| τ, ms | 19.6 ± 0.9 | 20.8 ± 2.3 | 17.2 ± 0.7 | 25.3 ± 3.0 | 4.5 | <0.01 | (1,2,3); (2,4) |
| I0, pA | 106 ± 8 | 52 ± 9 | 120 ± 7 | 60 ± 12 | 12.8 | <0.001 | (2,4); (1,3) |
| Sag, % | 9.7 ± 1.2 | 6.6 ± 0.9 | 20.2 ± 2.6 | 13.2 ± 2.0 | 9.1 | <0.001 | (1,2,4); (3) |
| RD, % | 8.9 ± 1.4 | 7.3 ± 0.9 | 19.0 ± 2.0 | 14.8 ± 2.4 | 8.8 | <0.001 | (1,2); (3; 4) |
| Hump, %) | 11.8 ± 1.9 | 8.6 ± 2.3 | 31.2 ± 3.7 | 32.3 ± 4.6 | 14.2 | <0.001 | (1,2); (3; 4) |
| TFS, ms | 136 ± 10 | 150 ± 20 | 117 ± 8 | 126 ± 17 | 1.2 | 0.31 | (1,2,3,4) |
| APT, mV | −40.4 ± 0.9 | −40.0 ± 0.9 | −41.4 ± 0.7 | −44 ± 0.8 | 3.1 | <0.05 | (1,2,3); (3,4) |
| APA, mV | 82 ± 2 | 64 ± 4 | 82 ± 2 | 78 ± 2 | 10.9 | <0.001 | (2); (1,3,4) |
| APD, ms | 0.93 ± 0.02 | 0.88 ± 0.04 | 0.90 ± 0.03 | 1.12 ± 0.11 | 3.3 | <0.05 | (1,2,3); (4) |
| AHP, mV | 11.9 ± 0.8 | 9.6 ± 1.0 | 10.4 ± 0.6 | 6.6 ± 1.0 | 5.9 | <0.01 | (4); (1,2,3) |
| fAHP, mV | 7.2 ± 1.5 | 5.5 ± 1.7 | 5.5 ± 1.1 | 6.0 ± 1.0 | 0.40 | 0.75 | (1,2,3,4) |
| ADP, mV | 0.84 ± 0.21 | 0.52 ± 0.28 | 2.4 ± 0.35 | n/a | 6.7 | <0.001 | (1,2); (3) |
| mAHP, mV | 5.5 ± 0.7 | 4.6 ± 0.9 | 7.3 ± 0.6 | n/a | 10.1 | <0.001 | (1,2); (3) |
| tAHP, ms | 37 ± 4 | 32 ± 6 | 61 ± 6 | 1.3 ± 0.2 | 19.8 | <0.001 | (4); (1,2); (3) |
| f1_k, Hz/pA | 0.42 ± 0.04 | 1.22 ± 0.15 | 0.70 ± 0.06 | 0.49 ± 0.07 | 19.4 | <0.001 | (1,4); (3,4); (2) |
| f1_threshold, Hz | 1.1 ± 1.2 | 1.0 ± 5.4 | 16.8 ± 7.7 | 102 ± 15 | 34.1 | <0.001 | (1,2,3); (4) |
| f2_k, Hz/pA | 0.25 ± 0.03 | 0.92 ± 0.17 | 0.33 ± 0.05 | 0.55 ± 0.07 | 14.6 | <0.001 | (1,3); (3,4); (2) |
| f2_threshold, Hz | 1.67 ± 0.65 | 0.52 ± 2.17 | −1.1 ± 1.1 | 39 ± 10 | 23.5 | <0.001 | (1,2,3); (4) |
| ISI1-2, ms | 215 ± 13 | 165 ± 28 | 186 ± 24 | 27 ± 15 | 13.2 | <0.001 | (4); (1,2,3) |
| FFAI, % | 37 ± 2 | 37 ± 5 | 66 ± 3 | 42 ± 3 | 21.7 | <0.001 | (1,2,4); (3) |
| FFAL, % | 50 ± 2 | 58 ± 5 | 76 ± 2 | 86 ± 2 | 34.4 | <0.001 | (1,2); (3); (4) |
| fss, Hz | 16.3 ± 0.7 | 26.6 ± 2.4 | 17.1 ± 0.9 | 16.5 ± 1.0 | 13.9 | <0.001 | (1,3,4); (2) |
Values are means ± SE of electrophysiological measurements in regular-spiking cells with low (RS_LRi; cluster 1; n = 27) and high input resistance (RS_HRi; cluster 2; n = 13), intermediate-spiking cells (IM; cluster 3; n = 24), and low-threshold-spiking cells (LTS; cluster 4; n = 13). See text for definition of electrophysiological characteristics. Following cluster analysis, Fisher's post hoc least significant difference (LSD) test was applied to compare the means for any pair of clusters 1–4 with statistically distinct properties; cluster numbers grouped within parentheses for a measurement indicate no significant difference between the indicated clusters, whereas different sets of parentheses for each measurement indicate significant differences between cells in each respective group of clusters.