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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Cancer Res. 2011 Nov 23;72(2):537–547. doi: 10.1158/0008-5472.CAN-11-1678

Figure 1. HAS2 gene is upregulated in CSCs from metastatic breast cancer cells.

Figure 1

(A) A heatmap was generated for the genes that were significantly up- or down-regulated at least 10 times in CSCs among 231BoM, 231BrM and MB231. (B) The expression of MMP1, HAS2, CRISPLD2 and OZD2 was examined by qRT-PCR for CSCs prepared from these three cell lines. The expression level of CSCs of MB231 was set as 0 in log10-scale (n=3). (C) HAS2 expressions in both parental cells (P) and CSCs (CSC) from each cell line were measured by qRT-PCR (n=3). (D) Western blotting for HAS2 protein in CSCs was performed.