Figure 10. The GFP and mRFP signals of tandem fluorescent LC3 (tfLC3, mRFP-GFP-LC3) show different localization patterns. HeLa cells were cotransfected with plasmids expressing either tfLC3 or LAMP1-CFP. Twenty-four hours after the transfection, the cells were starved in Hanks’ solution for 2 h, fixed and analyzed by microscopy. The lower panels are a higher magnification of the upper panels. Bar, 10 µm in the upper panels and 2 µm in the lower panels. Arrows in the lower panels point to (or mark the location of) typical examples of colocalized signals of mRFP and LAMP1. Arrowheads point to (or mark the location of) typical examples of colocalized particles of GFP and mRFP signals. This figure was previously published in reference 181 and is reproduced by permission of Landes Bioscience, copyright 2007.