Figure 14. Regulation of the SQSTM1 protein during autophagy. (A) The level of SQSTM1 during starvation. Atg5^+/+ and Atg5^−/− MEFs were cultured in DMEM without amino acids and serum for the indicated times, and then subjected to immunoblot analysis using anti-SQSTM1/p62 antibody (Progen Biotechnik). This figure was previously published in reference 25 and is reproduced by permission of Landes Bioscience, copyright 2007. (B) The level of SQSTM1 in the brain of neural cell-specific Atg5 knockout mice. This image was generously provided by Dr. Taichi Hara (Tokyo Medical and Dental University).