Figure 8. Movement of activated pDendra2-hp62 (orange) from the nucleus (middle) to the aggregate in ARPE-19 cells, revealed by confocal microscopy. Cells were exposed to 5 µM MG132 for 24 h to induce the formation of perinuclear aggregates.¹⁸⁵ The cells were then exposed to a UV pulse (the UV-induced area is shown by red lines that are inside of the nucleus) that converts Dendra2 from green to red, and the time shown after the pulse is indicated. SQSTM1/p62 is present in a small nuclear aggregrate, and is shuttled from the nucleus to a perinuclear large protein aggregate (detected as red). Scale bar, 5 µm. Image provided by K. Kaarniranta.