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. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Nanomedicine (Lond). 2012 Feb 23;7(4):521–539. doi: 10.2217/nnm.11.158

(A) Ocular cytokine mRNA levels post subretinal injection. Quantitative real-time PCR was performed on total RNA isolated from whole eyes at 6, 24 and 72 h post second subretinal injection with 1 μl saline containing 4.3 μg C-eGFP or L-eGFP as naked DNA or NPs. Saline alone and uninjected eyes were used as negative controls. Values depict the relative mRNA levels of cytokines normalized to β-actin.

eGFP: Enhanced GFP; C-eGFP: Circular-enhanced GFP; L-eGFP: Linear-enhanced GFP; NP: Nanoparticle. (B) Bio-Plex cytokine array to evaluate protein levels of ocular cytokines post second subretinal injection. Protein levels of ocular cytokines were evaluated in supernatants isolated from whole eyes treated as indicated in (A). Levels of acute and chronic phase cytokines (IL-6, IL-1 b, TNF-α and IFN-γ) were presented as mean ± standard deviation from three independent experiments that were run in triplicate and measured at the indicated time points post second injection. Eyes from untreated and subretinal injected animals with Bacillus cereus were respectively used as negative and positive controls. eGFP: Enhanced GFP; C-eGFP: Circular-enhanced GFP; L-eGFP: Linear-enhanced GFP; NP: Nanoparticle.

(A) Ocular cytokine mRNA levels post subretinal injection. Quantitative real-time PCR was performed on total RNA isolated from whole eyes at 6, 24 and 72 h post second subretinal injection with 1 μl saline containing 4.3 μg C-eGFP or L-eGFP as naked DNA or NPs. Saline alone and uninjected eyes were used as negative controls. Values depict the relative mRNA levels of cytokines normalized to β-actin.

eGFP: Enhanced GFP; C-eGFP: Circular-enhanced GFP; L-eGFP: Linear-enhanced GFP; NP: Nanoparticle. (B) Bio-Plex cytokine array to evaluate protein levels of ocular cytokines post second subretinal injection. Protein levels of ocular cytokines were evaluated in supernatants isolated from whole eyes treated as indicated in (A). Levels of acute and chronic phase cytokines (IL-6, IL-1 b, TNF-α and IFN-γ) were presented as mean ± standard deviation from three independent experiments that were run in triplicate and measured at the indicated time points post second injection. Eyes from untreated and subretinal injected animals with Bacillus cereus were respectively used as negative and positive controls. eGFP: Enhanced GFP; C-eGFP: Circular-enhanced GFP; L-eGFP: Linear-enhanced GFP; NP: Nanoparticle.