Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Nanomedicine (Lond). 2012 Feb 23;7(4):521–539. doi: 10.2217/nnm.11.158

Figure 7. Immunohistochemical assessment of infiltrating macrophages and neutrophils post second subretinal injection.

Figure 7

Figure 7

Immunohistochemical labeling of retinal sections obtained from mice injected with 1 μl saline containing 4.3 μg C-eGFP or L-eGFP as naked DNA or NPs (upper panels) and from untreated (negative control) and Bacillus cereus (positive control) injected eyes (lower panels). Analyses were performed at 6, 24, and 72 h post second injection and sections were labeled with (A) F4/80, a macrophage-specific marker.

C-eGFP: Circular enhanced GFP; GCL: Ganglion cell layer; INL: Inner nuclear layer; L-eGFP: Linear enhanced GFP; NP: Nanoparticle; ONL: Outer nuclear layer; OS: Outer segment.

(B)A neutrophil-specific marker (NIMP-14). Positive staining was seen in all cohorts with both markers at 6 h PI and disappeared at later time points. The positive staining cells are labeled in red. C-eGFP: Circular enhanced GFP; GCL: Ganglion cell layer; INL: Inner nuclear layer; L-eGFP: Linear enhanced GFP; NP: Nanoparticle; ONL: Outer nuclear layer; OS: Outer segment.