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. 2012 Apr 26;12:155. doi: 10.1186/1471-2407-12-155

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Copyright ©2012 Li et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Results of electrophoresis and Western blot.(A) PCR products of sKDR. Lane 1: sKDR; Lane 2: DNA Marker III. (B) Endonuclease digestion and PCR products. Lane 1: DNA Ladder; Lane 2: digestion of recombinant plasmid pTRKH2-PsT/sKDR; Lane 3: recombinant plasmid pTRKH2-PsT/sKDR; Lane 4: plasmid pTRKH2-PsT; Lane 5: PCR products of sKDR gene from recombinant plasmid pTRKH2-PsT/sKDR; Lane 6: PCR products of sKDR gene from recombinant plasmid pcDNA3.1/sKDR; Lane 7: DNA Marker. (C) RT-PCR and PCR products from recombinant Bifidobacterium infantis of pTRKH2-PsT/sKDR. Lane 1: PCR products of sKDR gene from recombinant plasmid pTRKH2-PsT/sKDR; Lane 2: RT-PCR product of RNA purified from Bifidobacterium infantis with pTRKH2-PsT/sKDR; Lane 3: DNA Marker. (D) Western blot analysis of sKDR expression. Lane 1: pTRKH2-PsT/sKDR; Lane 2: pTRKH2-PsT.