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. 2012 May 25;19(1):53. doi: 10.1186/1423-0127-19-53

Figure 3.

Figure 3

Knock down of NPM1 expression inhibited human colon cancer cell migration, invasion and proliferation. A. Western blotting analysis of NPM1 expression in HCT116 cells, HCT116 cells transfected with NPM1 specific siRNA and a control siRNA (Scr). β-actin was used as a loading control. B. MTT assay showed that the knockdown of NPM1 expression inhibited cell proliferation. Data were presented as mean ± SD. ***P < 0.0001. C. BrdU incorporation rate decreased after the knockdown of NPM1 in HCT116 colon cancer cells. **P < 0.001. D. PCNA expression in the nucleus was reduced after the down-regulation of NPM1. The PCNA staining index was calculated by counting the number of PCNA-positive staining cells per 100 cells in five randomly chosen areas. ***P < 0.0001. E. Knockdown of NPM1 decreased colon cancer cell invasion. Cell suspension with serum-free medium was loaded into the matrigel-coated upper chamber, and FBS-containing media were loaded into the lower chamber. The invaded cells were counted in three randomly chosen areas. *P < 0.05.