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. 2012 May 25;19(1):53. doi: 10.1186/1423-0127-19-53

Figure 4.

Figure 4

Exogenous expression of NPM1 enhanced colon cancer cell migration and invasive ability. A. Western blotting analysis of NPM1 expression in HT29 cells transfected with control or GFP-tagged NPM1 plasmid. NPM1 and GFP antibody were used to confirm the expression of NPM1. β-actin was used as a loading control. B. MTT assay showed that elevated NPM1 expression in human colon cancer HT29 cells increased cell proliferation. ***P < 0.0001. C. BrdU incorporation rate increased after exogenous NPM1 expression in HT29 cells. **P < 0.001. D. PCNA expression in the nucleus increased after exogenous NPM1 expression in HT29 cells. ***P < 0.0001. E. Elevated NPM1 expression in human colon cancer HT29 cells enhanced the cell invasive ability. **P < 0.001.