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. 2012 Jun 21;13:18. doi: 10.1186/1471-2199-13-18

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Copyright ©2012 Maier et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Domain analysis of PIM-1 – VDR interaction. (A) Yeast cells co-transformed with PIM-1-pAD-Gate2 and VDR-Fullength-pBD-Gate2 grow directly on PPI selective medium as well as yeast cells co-transformed with PIM-1-pAD-Gate2 and DBD-pBD-Gate2. Cells co-transformed with PIM-1-pAD-Gate2 and LBD-pBD-Gate2, PIM-1-pAD-Gate2 and pBD-Gate2, pAD-Gate2 and VDR-Fullength-pBD-Gate2, pAD-Gate2 and DBD-pBD-Gate2, or pAD-Gate2 and LBD-pBD-Gate2 show no growth on PPI selective medium. (B) All combination show strong growth on medium selective for positive transformation.