Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jun 21;13:18. doi: 10.1186/1471-2199-13-18

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2012 Maier et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Isolation of VDR-protein complex from human keratinocyte cell lysate. (A) Overview of the experimental procedure of the pull-down assay. (B) Western blot analysis with anti-PIM1 and anti-VDR antibody. PIM-1 and VDR were observed only in the lysate captured by the DRIP-2 peptide, while in the control lysate without peptide only unspecific lanes appeared. The expected sizes were for VDR about 48 kD, for PIM-1 about 34 kD and for the putative VDR/PIM-1 complex (unspecific band) respectively about 82 kD.