Skip to main content
. 2012 Jul 25;7(7):e41833. doi: 10.1371/journal.pone.0041833

Figure 2. Collagen and P4Hβ-expressing cells in the tumor.

Figure 2

A– Biopsies from panc2 subcutaneous tumors were stained with Sirius red for collagen (red) and immune-stained with P4Hβantibodies (red, indicated by arrows). Halofuginone treatment three times weekly at 15 µg/mouse resulted in major reductions in collagen content and in the total number of P4Hβ-expressing cells. B– Biopsies were double-immunostained with GFP-antibodies (green) and P4Hβ antibodies (red), and nuclei were stained with DAPI (blue). A stroma cell not expressing P4Hβ (white arrow) is adjacent to a tumor cell expressing P4Hβ (yellow arrow). C– Stroma cell expressing P4Hβ (white arrow). D– Halofuginone treatment reduced the total number of stroma cells expressing P4Hβ. Results are means ± SE and columns with different letters within each experiment differ significantly (P<0.05).