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. 2012 Jul 25;7(7):e41722. doi: 10.1371/journal.pone.0041722

Figure 1. Multiple sequence alignment and phylogenetic analysis of PTOX.

Figure 1

(A) Neighbour-joining phylogenetic dendrogram (spider) based upon an alignment of complete amino acid sequences of PTOX molecules. Grouping in different shades based upon structural and functional homology of PTOX polypeptides found in different species. Numbers at nodes indicate bootstrap confidence values (1000 replicates). The PTOX amino acid sequences used were from Arabidopsis thaliana GenBank accession number: CAA06190, tomato (Lycopersicon esculentum) GenBank accession number: AAG02286, rice (Oryza sativa; cultivar japonica) NCBI accession number: NP_001054199, wheat (Triticum aestivum) GenBank accession number: AAG00450, maize (Zea mays) NCBI accession number: NP_001150780, coffee (Coffea canephora) GenBank accession number: ABB70513, pepper (Capsicum annuum) GenBank accession number: AAG02288, Guillardia theta GenBank accession number: CAI77910, Chlamydomonas reinhardtii NCBI accession number: AF494290 (PTOX1) and NCBI accession number: XP_001703466 (PTOX2), Ostreococcus tauri (green alga) GenBank accession number: CAL58090, Bigelowiella natans (chlorarachniophytes) GenBank accession number: AAP79178, Anabaena variabilis ATCC 29413 (cyanobacteria) GenBank accession number: ABA21297, Nostoc sp. PCC 7120 (cyanobacteria) NCBI NP_486136, Prochlorococcus marinus subsp. Pastoris CCMP1986 (cyanobacteria) NCBI accession number: NP_892455, Prochlorococcus marinus Subsp. ASNC729 (cyanobacteria) Genbank accession number: ABE11017, Prochlorococcus marinus str. MIT 9312 (cyanobacteria) NCBI accession number: YP_396838, Prochlorococcus marinus str. NATL2A (cyanobacteria) NCBI accession number: YP_291624, Synechococcus sp. WH 8102 (cyanobacteria) NCBI accession number: NP_896980, Synechococcus sp. CC9902 (cyanobacteria) NCBI accession number: YP_376451, and Synechococcus sp. BL107 (cyanobacteria) NCBI: ZP_01468216. (B) Multiple sequence alignment of PTOX from Chlamydomonas and Arabidopsis. Conserved iron-binding residues are indicated by black arrows, whereas, exon 8 is boxed. Conserved sequences are shaded black. The transit peptide for At-PTOX is underlined.