Skip to main content
. 2012 Jul 25;7(7):e41722. doi: 10.1371/journal.pone.0041722

Figure 4. Immunodetection of Cr-PTOX1 protein by BN and 2D SDS-PAGE.

Figure 4

Thylakoid membranes from Cr-PTOXI-I or WT containing 4 µg chlorophyll were solubilised and separated by BN-PAGE. One of the BN-PAGE gel lanes was stained with Coomassie blue (A) and others were run in the second dimension by denaturing SDS-PAGE. One of the gels obtained was silver-stained (B) while other two were immunoblotted with anti-HA tag (C) or anti-D1 antibodies (D).