Figure 3. JAK-STAT5 signalling in Ikaros-rearranged ALL.

Western Blot analysis of STAT5 in IKZF1 deleted and wild-type leukemia primary cells (A) showed an increased phosphorylation of STAT5 in cells with Ikaros deletion. This was correlated with an increased production of Bcl-xl (B) as determined by RQ-PCR. (C) Western Blot analysis showed a marked increased phosphorylation of histone H2A.X in leukemia cells with Ikaros deletion compared to normal IKZF1 cells. Abbreviations: LC indicates leukemia cells. β-ACTIN was used as a loading control of the WB.