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. 2012 Jul 25;7(7):e42067. doi: 10.1371/journal.pone.0042067

Figure 3. VSL#3 and TNF modulate TJ protein expression in ilea from pre-inflamed SAMP mice.

Figure 3

mRNA transcript levels of TJ proteins (claudins 1–4, ZO-1, and occludin) were measured by qRT-PCR and expressed relative to ß-actin in full-thickness ilea from experimental mice following ex vivo culture with either VSL#3-CM or TNF. The greatest differences in expression were seen in claudin-2 (decrease) and occludin (increase) after either VSL#3-CM or TNF stimulation, and in claudin-3 (decrease) after TNF stimulation only. No differences in expression were noted for claudins 1, 4, and ZO-1 following exposure to either VSL#3-CM or TNF. *p<0.05 vs. vehicle cont. Results are representative of two independent experiments (N = 6/exp group) and expressed as mean ± SEM.