Table 1. List of MRM transitions.

			m/z (amu)
			PC		PE		PI		PG		PBut
transitions			Q1	Q3	Q1	Q3	Q1	Q3	Q1	Q3	Q1	Q3
16∶0/18∶1	a		804.5 → 281.2		716.5 → 281.2		835.5 → 281.2		747.5 → 281.2		729.6 → 281.2
	b		804.5 → 255.2		716.5 → 255.2		835.5 → 255.2		747.5 → 255.2		729.6 → 255.2
16∶0/18∶2	a		802.5 → 279.2		714.5 → 279.2		833.5 → 279.2		745.5 → 279.2		727.6 → 279.2
	b		802.5 → 255.2		714.5 → 255.2		833.5 → 255.2		745.5 → 255.2		727.6 → 255.2
16∶0/18∶3	a		800.5 → 277.2		712.5 → 277.2		831.5 → 277.2		743.5 → 277.2		725.6 → 277.2
	b		800.5 → 255.2		712.5 → 255.2		831.5 → 255.2		743.5 → 255.2		725.6 → 255.2
16∶1/18∶1	a		802.5 → 253.2		714.5 → 253.2		833.5 → 253.2		745.5 → 253.2		727.6 → 253.2
	b		802.5 → 281.2		714.5 → 281.2		833.5 → 281.2		745.5 → 281.2		727.6 → 281.2
16∶1/18∶2	a		800.5 → 279.2		712.5 → 279.2		831.5 → 279.2		743.5 → 279.2		725.6 →279.2
	b		800.5 → 253.2		712.5 → 253.2		831.5 → 253.2		743.5 → 253.2		725.6 → 253.2
16∶1/18∶3	a		798.5 → 277.2		710.5 → 277.2		829.5 → 277.2		741.5 → 277.2		723.6 →277.2
	b		798.5 → 253.2		710.5 → 253.2		829.5 → 253.2		741.5 → 253.2		723.6 → 253.2
18∶0/18∶1	a		832.6 → 281.2		744.6 → 281.2		863.6 → 281.2		775.6 → 281.2		757.6 → 281.2
	b		832.6 → 283.2		744.6 → 283.2		863.6 → 283.2		775.6 → 283.2		757.6 →283.2
18∶0/18∶2	a		830.6 → 279.2		742.6 → 279.2		861.6 → 279.2		773.6 → 279.2		755.6 → 279.2
	b		830.6 → 283.2		742.6 → 283.2		861.6 → 283.2		773.6 → 283.2		755.6 → 283.2
18∶0/18∶3	a		828.5 → 277.2		740.5 → 277.2		859.5 → 277.2		771.5 → 277.2		753.6 → 277.2
	b		828.5 → 283.2		740.5 → 283.2		859.5 → 283.2		771.5 → 283.2		753.6 → 283.2
18∶1/18∶1			830.6 → 281.2		742.6 → 281.2		861.6 → 281.2		773.6 → 281.2		755.6 → 281.2
18∶1/18∶2	a		828.5 → 279.2		740.5 → 279.2		859.5 → 279.2		771.5 → 279.2		753.6 → 279.2
	b		828.5 → 281.2		740.5 → 281.2		859.5 → 281.2		771.5 → 281.2		753.6 → 281.2
18∶1/18∶3	a		826.5 → 281.2		738.5 → 281.2		857.5 → 281.2		769.5 → 281.2		751.6→ 281.2
	b		826.5 → 277.2		738.5 → 277.2		857.5 → 277.2		769.5 → 277.2		751.6 → 277.2
18∶2/18∶2			826.5 → 279.2		738.5 → 279.2		857.5 → 279.2		769.5 → 279.2		751.6 → 279.2
18∶2/18∶3	a		824.5 → 277.2		736.5 → 277.2		855.5 → 277.2		767.5 → 277.2		749.6 → 277.2
	b		824.5 → 279.2		736.5 → 279.2		855.5 → 279.2		767.5 → 279.2		749.6 → 279.2
18∶3/18∶3			822.5 → 277.2		734.5 → 277.2		853.5 → 277.2		765.5 → 277.2		747.6 → 277.2

The list was established from PC, PI and PE enhanced resolution mass spectra. From the spectra the molecular species can be deduced as “carbon number:double bond”. The detailed fatty acid composition of the molecular species consistent with carbon number:double-bond formula can be assayed by transitions parent → product ions after CID of the ester bonds. In bold: m/z detected for PE, PC and PI by enhanced-resolution mass scanning. Underlined: transitions detected by product ion analysis. For PE and PI the parent ions m/z correspond to the [M−H]⁻ and for PC it corresponds to [M+HCOO]⁻. In agreement with formula a difference of 2 amu is noted between m/z of PE MRM (negative ionization M−H) and neutral loss of m/z 141 (positive ionization M+H).