Figure 1. Schematic representation of the vaccination protocol.

Three to five mice for each timepoint were injected at weeks 0 and 3 with the prime and at weeks 6 and 9 with the boost. Mice were either injected with saline (negative control, three mice per group), with protein plus alum alone, with protein plus a medium or high dose of IC31 alone, with protein plus alum and a medium or high dose of IC31, or with NYVAC-CN54 (five mice per each group per timepoint). At week 5, 11 or 15, a subset of mice were sacrificed and antibody and T-cell responses were analyzed ex vivo.