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. 2012 Jul 25;7(7):e42163. doi: 10.1371/journal.pone.0042163

Figure 4. Durable Env-CN54 gp140 specific CD8+ T-cell activation and cytokine secretion are detected following vaccination.

Figure 4

(A–C) Mice were primed with Env-CN54 gp140 in combination with alum alone, or with a medium (“med”) or high concentration of IC31 with or without alum, and then boosted with NYVAC-CN54 at three week intervals. A) Frequencies of activated CD8+ T cells as indicated by low CD62L expression at weeks 5, 11 and 15. Each data point corresponds to a single mouse in the experimental group. B) Percentage of CD8+ T cells secreting cytokines upon stimulation with the four peptide pools, as determined by ICS. C) Kinetic analysis of the frequencies of cytokine secreting CD8+ T cells following vaccination. Data points correspond to the medians of the frequencies obtained by peptide pool 1 stimulation. D–E) Mice were primed with NYVAC-CN54 and boosted with Env-CN54 gp140 in combination with adjuvants as indicated. D) Frequencies of activated CD8+ T cells as indicated by low CD62L expression at weeks 11 and 15. Each data point corresponds to a single mouse in the experimental group. E) Percentage of CD8+ T cells secreting cytokines at weeks 11 and 15. Labels are as shown in Figure 3. *p<0.05; **p<0.01.