Figure 4. Expression of IL-1beta, and HMGB1 associated with Th17 cell at 1 and 3 month after transplantation compared to before transplantation.

PBMC from patients before KT, patients at 1month after KT and patients at 3 month after KT were stimulated for 4 h ex vivo with PMA and ionomycin in the presence of GolgiStop. PBMC from all groups were treated as described in Figure 1 and Materials and Methods. The expression of IL-1beta (A), HMGB1(B) mRNA was measured using real-time PCR. Bars show the means.