Table 2. Binding of human monoclonal Abs 1_C02 and E05 to 309 HA and mutants.
| HA11 | Antigenic site | Kd, nM ± SD | |
| 1_C02 | E05 | ||
| 309 wt | Wild type | 1.0±0.2 | 2.0±0.1 |
| NNES121-124ITEG | A | 2.1±0.3 | 1.2±0.1 |
| N126T | 1.8±0.3 | 1.1±0.1 | |
| N133D | 0.8±0.2 | 0.6±0.3 | |
| TSSS135-138GSNA | 1.0±0.3 | 0.6±0.2 | |
| K140I | 1.0±0.4 | >1000 | |
| RSNNS142-146GPGSG | 1.3±0.4 | >1000 | |
| HL156-157KS | B | >1000 | 0.3±0.1 |
| KFK158-160GST | 6.0±3 | 0.8±0.2 | |
| NDQI189-192QEQT | 1.5±0.3 | 1.0±0.3 | |
| A196V | 1.1±0.2 | 1.9±0.1 | |
1
His6-tagged wildtype and mutant HA was expressed in insect cells and purified on a nickel column.