Figure 11. Oxidative and nitrostative challenge assays.

A) Oxidative stress response of phoB ^KP mutant by disc assay. The phoB ^KP mutant displayed greater sensitivity to 30% H₂O₂ (inhibition zone  = 32.5±0.5 mm) than the wild-type (inhibition zone  = 33±1.0 mm). B) The data is the means of measurements made in triplicate. *, Significant difference (P<0.05, Student t test). B) Effect of SNP (10 mM) on growth kinetics of WT and ΔphoB ^KP. C) Effect of NaNO₂ (10 mM) on growth profile of WT and ΔphoB ^KP.