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. 2012 Jul 25;7(7):e39685. doi: 10.1371/journal.pone.0039685

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FTIR spectra from a cluster of lag phase Rat-1 cells grown on a CaF₂ optical slide. Spectra were recorded (25×25 µm² aperture) over 160 minutes after washing the cells with DMEM prepared with 100% ²H₂O The initial spectrum (defined as t = 0) was used as the reference. The scale is the same as that used in Figure 2A . The horizontal line indicates that there is essentially no difference between the various spectra.