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. 2012 Jul 25;7(7):e40999. doi: 10.1371/journal.pone.0040999

Figure 5. AICAR induces Syk and c-Src activation, and enhances cell adhesion upon PMA stimulation.

Figure 5

(A) After treatment with agents as indicated, cell lysates were subjected to immunoblotting. (B) THP-1 cells were co-incubated with AICAR (0.1, 0.5 mM), A769662 (10 µM) and/or PMA (10–100 nM) for 4 h, followed by cell adhesion assay. (C) After expression of active or kinase dead AMPK, THP-1 cells were harvested and tested for the PMA (10, 100 nM)-induced cell adhesion. The expression and activity of AMPK were determined by immunobloting. *p<0.05 as compared to the PMA response without AICAR, A769662, or AMPK adenovirus infection. (D) THP-1 cells were infected with adenovirus expressing constitutively active or kinase-dead AMPK. After 48 h infection, cells were either stimulated with PMA (100 nM) or not for 30 min, and Syk and Src activities were determined by immunoblotting.