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. 2012 Jul 25;7(7):e41529. doi: 10.1371/journal.pone.0041529

Figure 4. VLP-specific IgA and IgG production in the reproductive tract.

Figure 4

Antibody levels were quantified in two sites in the reproductive tract following IN delivery of VLP and MB (25 µg, 100 µg, or 250 µg), MB alone (250 µg), VLP and alum delivered subcutaneously, VLP and CT delivered IN, or VLP alone delivered IN on day 0 and day 21. Vaginal lavages were collected from mice on days 0 (not shown), 12 (a), 21 (b), 42 (c), and 56 (d) and analyzed for VLP-specific IgA (A) and IgG (B) by ELISA. Uterine lavages were collected from mice on day 56 and analyzed for VLP-specific IgA (C) and IgG (D) by ELISA. Data is presented as the geometric mean titer (GMT). Error bars represent the standard errors of the means. Values that were significantly different from the values for the PBS control group are shown as: ΛP<0.05, *P<0.01, **P<0.001. Samples from the PBS group of mock-vaccinated mice were negative by ELISA (not shown). The horizontal hatched line indicates the limit of detection for the assay.