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. 2012 Jul 25;7(7):e41529. doi: 10.1371/journal.pone.0041529

Figure 5. MB induces a sustained systemic immune response.

Figure 5

(A) Timeline of study 2 vaccinations and sample collection days. The humoral immune response was measured and following IN delivery of VLP and MB (200 µg), VLP and GARD (25 µg), or VLP alone on day 0 and day 21 in an extended study (day 112). (B) Serum samples were collected on days 0 (not shown), 12 (a), 21 (b), 42 (c), 56 (d), 84 (e), and 112 (f), analyzed for VLP-specific IgG by ELISA and presented as the geometric mean titer (GMT). (C) On day 112, splenocytes were isolated and pooled into 3 groups to determine the frequency of VLP-specific IgG-secreting cells by ELISPOT. (D) Serum samples were collected on days 0 (not shown), 12 (a), 21 (b), 42 (c), 56 (d), 84 (e), and 112 (f), analyzed for VLP-specific IgA by ELISA and presented as the geometric mean titer (GMT). Values that were significantly different from the values for the PBS control group are shown as: ΛP<0.05, *P<0.01, **P<0.001. Samples from the PBS group of mock-vaccinated mice were negative by ELISA (not shown). The horizontal hatched line indicates the limit of detection for the assay.