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. 2012 Jul 25;7(7):e41529. doi: 10.1371/journal.pone.0041529

Figure 6. MB induces a sustained mucosal immune response at distal mucosal sites.

Figure 6

VLP-specific antibody production was measured at reproductive, oral, and respiratory sites following IN delivery of VLP and MB (200 µg), VLP and GARD (25 µg), or VLP alone on day 0 and day 21 in an extended study (day 112). (A) Vaginal lavages were collected on days 0 (not shown), 12 (a), 21 (b), 42 (c), 56 (d), 84 (e), and 112 (f) and analyzed for VLP-specific IgA by ELISA. (B) Uterine, salivary, and nasal samples were collected on day 112 and analyzed for VLP-specific IgA by ELISA. Values that were significantly different from the values for the PBS control group are shown as: ΛP<0.05, *P<0.01, **P<0.001. Samples from the PBS group of mock-vaccinated mice were negative by ELISA (not shown). The horizontal hatched line indicates the limit of detection for the assay.