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A: pre-pupae workers’ hind leg; B: white-eyed pupae workers’ hind leg; C: pre-pupae queens’ hind leg; D: white-eyed pupae queens’ hind leg. In blue: DAPI; in red: Ubx, note that the different red degrees represent the Ubx level observed in the stainings; Tar: tarsi; Btar: basitarsi; Tb: tibia; Fm: femur; Cx-Tr: coxa and trochanter.
